The club's number one goalkeeper Marc-Andre ter Stegen is a major doubt for the contest due to a back problem which he picked up during a training session with Germany over the international break.
As a result, number two Pena could make just his third league appearance for the club, with the La Liga champions unlikely to take any risks when it comes to Ter Stegen's fitness.
Gavi, meanwhile, will miss the rest of the campaign due to the ACL injury that he suffered while turning out for Spain in their Euro 2024 qualification clash with Georgia.
Alejandro Balde started on the bench against Alaves before the international break, but he is set to return to the XI this weekend.
Barcelona possible starting lineup: Pena; Cancelo, Araujo, Kounde, Balde; Gundogan, Pedri, F Lopez; Yamal, Lewandowski, FelixNo Data Analysis info